INVESTIGATION OF THE EFFECT OF DIFFERENT SOLVENT RATIOS IN THE CHEMOMETRIC PHYTO-EXTRACTION AND IN-VITRO SCREENING OF Carica papaya FRUIT, USING RESPONSE SURFACE METHODOLOGY [RSM]

Carica papaya plant is a popular medicinal with notable anti-parasitic potentials. This work reports chemometrics of the three solvents [petroleum ether, chloroform and methanol] in in-vitro screening Plasmodium falciparum Trypanosome brucei b. The Response Surface Methodology [RSM] method based on second order variables Box-Behnken Design (BBD) was employed to obtain optimal combination extraction conditions. process parameters fruit were optimized by BBD so as get maximal yield bio-actives. Different solvent ratios used achieve different response chemometric. For b., extracts subjected assay procedures see their viability. Summarily, 3D surface plot contour derived from mathematical models applied determine Under conditions, experimental value 77.97% viability f. close agreement predicted (77.97 ± 0.01 %) model 82.86 1.10%was for an experimented 83.48%; showing correlation between values. Considering RSM studies, it found that mixture petroleum methanol at ratio 55 mL: 27.50 17.50 mL gave optimum screening. Hence, polarity affects bioactive compounds material.